Towards a Database of Breast Cancer Proteomics: Proteome Profiling of Human Breast Cancer Showed Unique Pattern of Protein Expression and Phosphorylation

Serhiy Souchelnytskyi, Associate Professor, Head of Laboratory, Karolinska University Hospital

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Launch presentation

About the speaker

Dr. Souchelnytskyi graduated from the Lviv State University, Lviv, Ukraine, 1985, in biochemistry. Dr. Souchelnytskyi received PhD at the Institute of Biochemistry, National academy of Sciences of Ukraine, Lviv. After a post-doc at the INSERM U244, Grenoble, France, and a group leader position at the Ludwig Institute for Cancer Research in Uppsala, Sweden, Dr. Souchelnytskyi is working at the Karolinska Biomics Centre, Stockholm, Sweden.

Scientific interests of Dr. Souchelnytskyi are in studies of carcinogenic transformation. Proteomics and systems biology are main tools used to understand proteome changes during carcinogenesis. Development of specific inhibitors to manipulate transforming growth factor-beta signalling and proteomics-based search for markers of human breast cancer are translation-oriented projects in group of Dr. Souchelnytskyi.

Abstract

We perform proteome profiling of tumour and non-cancerous tissues, primary and established human breast epithelial cells. We generate a database which currently includes more than 400 proteins. These proteins were identified by us as differentially expressed or phosphorylated in cancer, as compared to non-cancerous conditions. Analysis of the identified proteins showed that multiple regulatory pathways were affected, e.g. metabolism, apoptosis, stress response, serine/threonine and tyrosine kinase signalling. Systemic exploration of identified proteins indicated that marker values can be assigned to the pattern of status of signalling pathways, e.g. combination of active and inactive pathways. Experimental approaches and results of the pathway marker search will be discussed.

Launch presentation